Khamernebti I was a daughter of Kheops. The name of here mother is not known. Her marriage to her (half ?) brother Khefren is believed by some to have reinforced the latter's position on the throne. However, if this marriage were indeed meant to reinforce Khefren's claims to the throne, then it remains unclear why Khefren's brother and predecessor Djedefre didn't marry Khamernebti I or why Khefren didn't marry Djedefre's widow-sister Hetepheres II.
Khamernebti I bore her husband at least two children: Mykerinos and Khamernebti II.